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Cell Signaling Technology Inc rabbit anti phospho ensa
Rabbit Anti Phospho Ensa, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti+phospho+ensa/Phospho-ENSA+(Ser67)%2FARPP19+(Ser62)+Antibody/pm40447768-145-41-72
Average 94 stars, based on 23 article reviews

rabbit anti phospho ensa - by Bioz Stars, 2026-09

94/100 stars

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Article Title: Unveiling an Arpp-19 phosphorylation switch that grants chromosome stability.
Article Snippet: Primary antibodies used in this study: mouse anti-α-tubulin (Cat# T9026, clone DM1A, Sigma-Aldrich; 1:1000); mouse anti-Cdk1 (Cat# sc-54, Santa Cruz Biotechnology; 1:1000), rabbit anti-cyclin B1(Cat# A305-000A-M, Bethyl Laboratories; 1:1000), rabbit anti-Arpp-19 (Cat# 11678-1-AP, Proteintech; 1:1000), rabbit anti-Ensa (Proteintech; Cat 14518-1-AP; 1:1000), rabbit anti-phospho-Ensa (Ser67)/Arpp-19(Ser62) (DpSG;Cat# 5240S,Cell Signaling Technology; 1:1000), rabbit anti-Fcp1 (Cat# A301-172A; Bethyl Laboratories; 1:1000), human anti-centromere (CREST; Cat# 15-234, Antibodies Incorporated; 1:100), rabbit anti-Cdk substrate motif [(K/H) pSP] (Cat# 9477, Cell Signaling Technology; 1:1000), anti-Flag Peroxidase (HRP) (Cat# A8592, Sigma-Aldrich; 1:500), mouse anti-γ-tubulin (Cat# T5326, Clone GTU-88, Sigma-Aldrich; 1:1000), rabbit anti-Cdc25C (Cat# 4688S, Cell Signaling Technology; 1:1000), anti-Flag FITC (Cat# F4049, Sigma-Aldrich; 1:200).. Rabbit polyclonal antibody against phosphorylated serine 23 of human Arpp-19 (rabbit anti-phospho-serine-23-Arpp-19; pS23-Arpp-19) was raised using C-EDKVT(Sp)PEK-coNH2 peptide as immunogen; peptide synthesis, rabbit inoculation, serum production and cross-affinity antibody purification were carried out by CovalAb (Bron, France; 1:100).

Article Title: Unveiling an Arpp-19 phosphorylation switch that grants chromosome stability
Article Snippet: Primary antibodies used in this study: mouse anti-α-tubulin (Cat# T9026, clone DM1A, Sigma-Aldrich; 1:1000); mouse anti-Cdk1 (Cat# sc-54, Santa Cruz Biotechnology; 1:1000), rabbit anti-cyclin B1(Cat# A305-000A-M, Bethyl Laboratories; 1:1000), rabbit anti-Arpp-19 (Cat# 11678-1-AP, Proteintech; 1:1000), rabbit anti-Ensa (Proteintech; Cat 14518-1-AP; 1:1000), rabbit anti-phospho-Ensa (Ser67)/Arpp-19(Ser62) (DpSG ; Cat# 5240S, Cell Signaling Technology; 1:1000), rabbit anti-Fcp1 (Cat# A301-172A; Bethyl Laboratories; 1:1000), human anti-centromere (CREST; Cat# 15-234, Antibodies Incorporated; 1:100), rabbit anti-Cdk substrate motif [(K/H)pSP] (Cat# 9477, Cell Signaling Technology; 1:1000), anti-Flag Peroxidase (HRP) (Cat# A8592, Sigma-Aldrich; 1:500), mouse anti-γ-tubulin (Cat# T5326, Clone GTU-88, Sigma-Aldrich; 1:1000), rabbit anti-Cdc25C (Cat# 4688S, Cell Signaling Technology; 1:1000), anti-Flag FITC (Cat# F4049, Sigma-Aldrich; 1:200).. Rabbit polyclonal antibody against phosphorylated serine 23 of human Arpp-19 (rabbit anti-phospho-serine-23-Arpp-19; pS23-Arpp-19) was raised using C-EDKVT(Sp)PEK-coNH2 peptide as immunogen; peptide synthesis, rabbit inoculation, serum production and cross-affinity antibody purification were carried out by CovalAb (Bron, France; 1:100).

Article Title: Harnessing transcriptionally driven chromosomal instability adaptation to target therapy-refractory lethal prostate cancer
Article Snippet: Rabbit anti-MASTL (IHC) , Abcam , Cat# ab86387; RRID:AB1925198.Rabbit anti-MASTL (IHC) , Abcam , Cat# ab86387; RRID:AB1925198.. Rabbit anti-phospho-ENSA (Ser67)/ARPP19 (Ser62) , Cell Signaling , Cat# 5240; RRID:AB11220425.. Rabbit polyclonal anti-ENSA , Cell Signaling , Cat# 8770; RRID:AB11217626.Rabbit polyclonal anti-ENSA , Cell Signaling , Cat# 8770; RRID:AB11217626.

Recombinant:

SYBR Green Assay:

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A DSG (red) and the KKR (violet) motifs, and the triple K36A/K38A/R40A (KKR/AAA) Xenopus Arpp19 alanine mutant. Table depicting dephosphorylation time, B55-Arpp19 interaction and the capacity to promote mitotic entry in Arpp19-depleted extracts. B 50 ng of wild type or KKR/AAA Arpp19 mutant phosphorylated ‘in vitro’ by GwlK72M was supplemented to kinase-inactivated extracts depleted or not of the B55 protein. Arpp19 levels and of S67/S71 phosphorylation were analysed by western blotting and autoradiography, respectively. The 33 P-Arpp19/western blotting Arpp19 signal ratios were calculated using ImageJ for each time point. The percentage of the ratio remaining at each time point with respect to the ratio at 0 min was calculated and represented in bar graphs as the mean percentage ± SD; n = 3 biological independent samples. C A His-Arpp19 pulldown equivalent to 20 ng of wild type or KKR/AAA mutant was submitted to western blotting, and the amount of B55 and the levels of Arpp19 bound to the beads shown. B55/Arpp19 signal ratios were calculated using ImageJ and represented in a bar graph as the mean ratio ± SD; n = 5 biological independent samples. D Arpp19-depleted extracts were supplemented with human GwlK72M and a wild type or a KKR/AAA Arpp19 mutant and phosphorylation of Human Gwl, of Tyr15, of Cdk1 and Arpp19 ectopic levels (His-Arpp19) were assessed. E A schematic of DSG regions indicating residues mutated into alanine or threonine. Table representing results on the S67/S71 dephosphorylation time and the capacities to bind B55 or to restore mitotic entry in Arpp19-depleted egg extracts of each Arpp19 form. F Wild-type Arpp19 and the indicated mutants of the DSG motif were ‘in vitro’ phosphorylated by hGwlK72M and 1 µl sample removed at 10 and 40 min, to measure S67/S71 phosphorylation by autoradiography ( P33 Arp). The amount of Arpp19 was assessed by Coomassie blue staining (Arp) 33 . P-Arpp19 levels were normalized by Arpp19 amount using Coomassie blue signal and the increase in phosphorylation between time 10 and 40 min calculated. Data from three different experiments was then used to obtain the mean ± SD and represented in a bar graph; n = 3 biological independent samples.

Journal: Nature Communications

Article Title: The study of the determinants controlling Arpp19 phosphatase-inhibitory activity reveals an Arpp19/PP2A-B55 feedback loop

doi: 10.1038/s41467-021-23657-0

Figure Lengend Snippet: A DSG (red) and the KKR (violet) motifs, and the triple K36A/K38A/R40A (KKR/AAA) Xenopus Arpp19 alanine mutant. Table depicting dephosphorylation time, B55-Arpp19 interaction and the capacity to promote mitotic entry in Arpp19-depleted extracts. B 50 ng of wild type or KKR/AAA Arpp19 mutant phosphorylated ‘in vitro’ by GwlK72M was supplemented to kinase-inactivated extracts depleted or not of the B55 protein. Arpp19 levels and of S67/S71 phosphorylation were analysed by western blotting and autoradiography, respectively. The 33 P-Arpp19/western blotting Arpp19 signal ratios were calculated using ImageJ for each time point. The percentage of the ratio remaining at each time point with respect to the ratio at 0 min was calculated and represented in bar graphs as the mean percentage ± SD; n = 3 biological independent samples. C A His-Arpp19 pulldown equivalent to 20 ng of wild type or KKR/AAA mutant was submitted to western blotting, and the amount of B55 and the levels of Arpp19 bound to the beads shown. B55/Arpp19 signal ratios were calculated using ImageJ and represented in a bar graph as the mean ratio ± SD; n = 5 biological independent samples. D Arpp19-depleted extracts were supplemented with human GwlK72M and a wild type or a KKR/AAA Arpp19 mutant and phosphorylation of Human Gwl, of Tyr15, of Cdk1 and Arpp19 ectopic levels (His-Arpp19) were assessed. E A schematic of DSG regions indicating residues mutated into alanine or threonine. Table representing results on the S67/S71 dephosphorylation time and the capacities to bind B55 or to restore mitotic entry in Arpp19-depleted egg extracts of each Arpp19 form. F Wild-type Arpp19 and the indicated mutants of the DSG motif were ‘in vitro’ phosphorylated by hGwlK72M and 1 µl sample removed at 10 and 40 min, to measure S67/S71 phosphorylation by autoradiography ( P33 Arp). The amount of Arpp19 was assessed by Coomassie blue staining (Arp) 33 . P-Arpp19 levels were normalized by Arpp19 amount using Coomassie blue signal and the increase in phosphorylation between time 10 and 40 min calculated. Data from three different experiments was then used to obtain the mean ± SD and represented in a bar graph; n = 3 biological independent samples.

Article Snippet: The antibodies used in this study are the following: Rabbit Polyclonal anti-Human Gwl , Rabbit Polyclonal Phospho-Cdc2 (Tyr15) (Cell Signaling Technology Cat#9111), Rabbit Polyclonal anti- Xenopus Arpp19 , Rabbit Polyclonal anti-PP2A/B55δ (Cell Signaling Technology Cat#2290), Rabbit Polyclonal anti- Xenopus Cdc27 , Rabbit Polyclonal anti- Xenopus Cyclin B2 , Rabbit Polyclonal anti- Xenopus Cdk1 , Rabbit Monoclonal PhosphoThr320 of PP1 (Abcam Cat#62334), Mouse Monoclonal Phospho-Erk (Cell Signaling Cat# 9106 S), Rabbit Polyclonal anti-phosphorylated Arpp19 (S67) (Cell Signaling Cat#5240 S), Rabbit Polyclonal anti-PRC1 (Santa Cruz Cat# 376982), Goat Polyclonal anti-phosphorylated PRC1 (T481) (Santa Cruz Cat#11768), Mouse Monoclonal anti-PP2A (C) subunit-α (isoform Merck Millipore Cat#05-42), Rat Polyclonal PP2A (A) subunit (Cell Signaling Cat#2260), Mouse Monoclonal anti-PP1 kindly gifted by Dr M Bollen and used in Ma et al. , Anti-PP4c (Bethyl Cat#A300-835A), Anti-PP6 (Santa Cruz Cat#393294), Goat anti-rat IgG-horseradish peroxidase (HRP) (Santa Cruz Cat#2006), HRP-conjugated anti-Rabbit secondary antibodies (Cell Signalling Technology Cat#7074), Donkey anti-goat IgG-HRP (Santa Cruz Cat# sc-2020), Rabbit Polyclonal anti-phosphorylated Arpp19 (S109/S113) (this study), and Rabbit Polyclonal anti-PP2A (B56) γ-subunit (this study).

Techniques: Mutagenesis, De-Phosphorylation Assay, In Vitro, Phospho-proteomics, Western Blot, Autoradiography, Staining

A S67/S71 dephosphorylation of wild-type Arpp19 or of the indicated DSG Arpp19 mutants was measured in kinase-inactivated extracts depleted or not of B55, as well as upon the addition of a purified PP2A-B55 phosphatase by autoradiography ( P33 Arp). The amount of Arpp19 in each sample was assessed by western blot (Arp). The percentage of phosphorylation remaining with respect to the starting point calculated as in Fig. and represented in bar graph as the mean value ± SD; n = 3 biological independent samples. B Dephosphorylation of the wild type and the indicated mutants of Arpp19 were performed in kinase-inactivated extracts and quantified in a bar graph as the mean value ± SD; n = 3 biological independent samples.

Journal: Nature Communications

Article Title: The study of the determinants controlling Arpp19 phosphatase-inhibitory activity reveals an Arpp19/PP2A-B55 feedback loop

doi: 10.1038/s41467-021-23657-0

Figure Lengend Snippet: A S67/S71 dephosphorylation of wild-type Arpp19 or of the indicated DSG Arpp19 mutants was measured in kinase-inactivated extracts depleted or not of B55, as well as upon the addition of a purified PP2A-B55 phosphatase by autoradiography ( P33 Arp). The amount of Arpp19 in each sample was assessed by western blot (Arp). The percentage of phosphorylation remaining with respect to the starting point calculated as in Fig. and represented in bar graph as the mean value ± SD; n = 3 biological independent samples. B Dephosphorylation of the wild type and the indicated mutants of Arpp19 were performed in kinase-inactivated extracts and quantified in a bar graph as the mean value ± SD; n = 3 biological independent samples.

Techniques: De-Phosphorylation Assay, Purification, Autoradiography, Western Blot, Phospho-proteomics

A B55 levels associated to 20 ng of wild type or the indicated DSG mutants of His-Arpp19-pulldowns. Arpp19 amount in these pulldowns is also shown. Data were represented as mean B55/Arpp19 ratio ± SD. Two-tailed unpaired Student’s t -tests were performed in each pulldown to determine statistical relevance. p vs. wild-type Arpp19 is shown; n = 3 biological independent samples for mutants Y68A, G72A and Y74A; n = 5 for the D73A, n = 6 for the wild-type form and n = 4 for the rest. B The wild type and the indicated DSG mutant forms of Arpp19 were thio-phosphorylated and used for His-pulldown. B55 and Arpp19 levels were checked by western blotting and shown. The B55/Arpp19 ratios were quantified and represented in a bar graph as mean ± SD. Two-tailed unpaired Student t -tests were performed in each pulldown to determine statistical relevance. p vs. wild-type Arpp19 is shown; n = 3 biological independent samples. C The B55/Arpp19 ratios were obtained as in A for the indicated mutants and represented in a bar graph as mean ± SD. Two-tailed unpaired; p vs. wild-type Arpp19 is shown; n = 3 biological independent samples for the wild-type form; n = 8 for the G72A mutant, n = 5 for the G72A-S71A mutant and n = 3 for the G72A-S71T mutant. D Prophase oocytes were injected or not (PG) with 50 ng of the wild-type His-Arpp19 protein or with the indicated mutant forms and, 1 h later, treated with progesterone. GVBD was then scored as a function of time. Germinal vesicle (GV) and mature oocytes (GVBD) were western blotted to determine the levels of the injected protein, as well as the phosphorylation of Gwl and of the inhibitory site of Cdk1 Tyrosine 15. E As for E , except that the indicated mutant form of Arpp19 was used.

Journal: Nature Communications

Article Title: The study of the determinants controlling Arpp19 phosphatase-inhibitory activity reveals an Arpp19/PP2A-B55 feedback loop

doi: 10.1038/s41467-021-23657-0

Figure Lengend Snippet: A B55 levels associated to 20 ng of wild type or the indicated DSG mutants of His-Arpp19-pulldowns. Arpp19 amount in these pulldowns is also shown. Data were represented as mean B55/Arpp19 ratio ± SD. Two-tailed unpaired Student’s t -tests were performed in each pulldown to determine statistical relevance. p vs. wild-type Arpp19 is shown; n = 3 biological independent samples for mutants Y68A, G72A and Y74A; n = 5 for the D73A, n = 6 for the wild-type form and n = 4 for the rest. B The wild type and the indicated DSG mutant forms of Arpp19 were thio-phosphorylated and used for His-pulldown. B55 and Arpp19 levels were checked by western blotting and shown. The B55/Arpp19 ratios were quantified and represented in a bar graph as mean ± SD. Two-tailed unpaired Student t -tests were performed in each pulldown to determine statistical relevance. p vs. wild-type Arpp19 is shown; n = 3 biological independent samples. C The B55/Arpp19 ratios were obtained as in A for the indicated mutants and represented in a bar graph as mean ± SD. Two-tailed unpaired; p vs. wild-type Arpp19 is shown; n = 3 biological independent samples for the wild-type form; n = 8 for the G72A mutant, n = 5 for the G72A-S71A mutant and n = 3 for the G72A-S71T mutant. D Prophase oocytes were injected or not (PG) with 50 ng of the wild-type His-Arpp19 protein or with the indicated mutant forms and, 1 h later, treated with progesterone. GVBD was then scored as a function of time. Germinal vesicle (GV) and mature oocytes (GVBD) were western blotted to determine the levels of the injected protein, as well as the phosphorylation of Gwl and of the inhibitory site of Cdk1 Tyrosine 15. E As for E , except that the indicated mutant form of Arpp19 was used.

Techniques: Two Tailed Test, Mutagenesis, Western Blot, Injection, Phospho-proteomics

A Represented in dashed lines are the sequences deleted in the specified Arpp19 mutants. A table summarizing data of the S67/S71 dephosphorylation time in kinase-inactivated extracts as well as the binding or not to B55 and the capacity to restore the mitotic state in Arpp19-depleted extracts of all these mutants is also shown. Yellow and orange lines denote a dephosphorylation time of S67/S71 of <1 min or between 2 to 4 min, respectively. B The dephosphorylation of S67/S71 of (48–120) Arpp19 mutant was assayed in kinase-inactivated extracts and revealed by autoradiography. The amount of this Arpp19 mutant form in each sample is also shown. Bar graph shows mean percentage of phosphorylation remaining respect to the one of the starting point ± SD; n = 3 biological independent samples. C A volume of His-Arpp19 pulldown sample corresponding to 20 ng of wild type or (48–120) mutant is submitted to western blot and the associated B55 protein as well as the amount of Arpp19 present in the beads shown. Due to the insolubility of the His-Arpp19 (48–120) mutant, we used a double-tagged MBP-His-Arpp19. Quantification of the B55/Arpp19 ratio of three experiments was performed and represented as mean ratio ± SD; n = 3 biological independent samples. D S67/S71 dephosphorylation assays of the indicated Arpp19 mutants was tested in kinase-inactivated extracts depleted or not of B55 and revealed by autoradiography. The amounts of Arpp19 mutant proteins present in each sample was checked by western blotting. Results of three experiments are quantified and represented as the mean percentage of phosphorylation remaining ± SD; n = 3 biological independent samples. E Levels of B55 associated to a volume of beads equivalent to 20 ng of the wild type and the indicated C-terminal Arpp19 mutants. The amount of these mutants bound to the beads is also shown. The mean B55/Arpp19 and SD was calculated from three experiments and represented as a bar graph. Two-tailed unpaired Student’s t -tests were performed in each pulldown to determine statistical relevance. p vs. wild-type Arpp19 is shown; n = 5 biological independent samples for mutants (1–75) and (1–86), and n = 6 for the rest.

Journal: Nature Communications

Article Title: The study of the determinants controlling Arpp19 phosphatase-inhibitory activity reveals an Arpp19/PP2A-B55 feedback loop

doi: 10.1038/s41467-021-23657-0

Figure Lengend Snippet: A Represented in dashed lines are the sequences deleted in the specified Arpp19 mutants. A table summarizing data of the S67/S71 dephosphorylation time in kinase-inactivated extracts as well as the binding or not to B55 and the capacity to restore the mitotic state in Arpp19-depleted extracts of all these mutants is also shown. Yellow and orange lines denote a dephosphorylation time of S67/S71 of <1 min or between 2 to 4 min, respectively. B The dephosphorylation of S67/S71 of (48–120) Arpp19 mutant was assayed in kinase-inactivated extracts and revealed by autoradiography. The amount of this Arpp19 mutant form in each sample is also shown. Bar graph shows mean percentage of phosphorylation remaining respect to the one of the starting point ± SD; n = 3 biological independent samples. C A volume of His-Arpp19 pulldown sample corresponding to 20 ng of wild type or (48–120) mutant is submitted to western blot and the associated B55 protein as well as the amount of Arpp19 present in the beads shown. Due to the insolubility of the His-Arpp19 (48–120) mutant, we used a double-tagged MBP-His-Arpp19. Quantification of the B55/Arpp19 ratio of three experiments was performed and represented as mean ratio ± SD; n = 3 biological independent samples. D S67/S71 dephosphorylation assays of the indicated Arpp19 mutants was tested in kinase-inactivated extracts depleted or not of B55 and revealed by autoradiography. The amounts of Arpp19 mutant proteins present in each sample was checked by western blotting. Results of three experiments are quantified and represented as the mean percentage of phosphorylation remaining ± SD; n = 3 biological independent samples. E Levels of B55 associated to a volume of beads equivalent to 20 ng of the wild type and the indicated C-terminal Arpp19 mutants. The amount of these mutants bound to the beads is also shown. The mean B55/Arpp19 and SD was calculated from three experiments and represented as a bar graph. Two-tailed unpaired Student’s t -tests were performed in each pulldown to determine statistical relevance. p vs. wild-type Arpp19 is shown; n = 5 biological independent samples for mutants (1–75) and (1–86), and n = 6 for the rest.

Techniques: De-Phosphorylation Assay, Binding Assay, Mutagenesis, Autoradiography, Phospho-proteomics, Western Blot, Two Tailed Test

A Schematic representation of the ‘inter-cassette’ (sequence 78–95) and the ‘cassette’ (sequence 96–111) deleted regions of Arpp19, as well as the residues on the ‘cassette motif’ that have been mutated into alanine. B Dephosphorylation of S67/S71 of the indicated Arpp19 mutants in kinase-inactivated extracts devoid or not of B55. The amount of Arpp19 in each sample was assessed by western blotting. Data were quantified and represented as the mean percentage of phosphorylation remaining ± SD; n = 3 biological independent samples. C Western blotting showing the association of B55 to the indicated mutants of Arpp19 as well as the quantification of B55/Arpp19 mean ratio ± SD. Two-tailed unpaired Student’s t -tests were performed in each pulldown to determine statistical relevance. p vs. wild-type Arpp19 is shown; n = 3 biological independent samples for mutants D(78–95), D(96–111) and 2 A; n = 6 for the wild-type form and n = 4 for the 6A mutant. D Scheme depicting the two regions that have been deleted in the ‘inter-cassette’ motif. Table illustrating data on S67/S71 dephosphorylation timing as well as the capacity to bind B55 and to restore mitosis of the two mutants. Western blotting showing the amount of B55 present in the His-App19 pulldown assays of the indicated Arpp19 mutant forms. Graph bar representing the mean B55/Arpp19 ratio ± SD; n = 3 biological independent samples. E S67/S71 dephosphorylation assays of the indicated mutants of Arpp19 in kinase-inactivated extracts devoid or not of B55. Results were quantified and represented as the mean percentage of phosphorylation remaining ± SD; n = 3 biological independent samples. F A schematic of the DSG (D1) and the cassette (D2) regions of Arpp19 that have been exchanged in the D2-D1 mutant. A table with the dephosphorylation, binding and rescue results is also shown. The association of B55 to the D2-D1 mutant compared to the wild-type Arpp19 and the quantification of the mean B55/Arpp19 ± SD are shown. Two-tailed unpaired Student’s t -tests were performed in each pulldown to determine statistical relevance. p vs. wild-type Arpp19 is shown; n = 3 biological independent samples. G Dephosphorylation assay of S67/S71 of the wild type and the D2-D1 mutant in kinase-inactivated extracts that have been depleted or not of B55. Data from three experiments were represented as the mean percentage of phosphorylation remaining ± SD. n = 3 biological independent samples.

Journal: Nature Communications

Article Title: The study of the determinants controlling Arpp19 phosphatase-inhibitory activity reveals an Arpp19/PP2A-B55 feedback loop

doi: 10.1038/s41467-021-23657-0

Figure Lengend Snippet: A Schematic representation of the ‘inter-cassette’ (sequence 78–95) and the ‘cassette’ (sequence 96–111) deleted regions of Arpp19, as well as the residues on the ‘cassette motif’ that have been mutated into alanine. B Dephosphorylation of S67/S71 of the indicated Arpp19 mutants in kinase-inactivated extracts devoid or not of B55. The amount of Arpp19 in each sample was assessed by western blotting. Data were quantified and represented as the mean percentage of phosphorylation remaining ± SD; n = 3 biological independent samples. C Western blotting showing the association of B55 to the indicated mutants of Arpp19 as well as the quantification of B55/Arpp19 mean ratio ± SD. Two-tailed unpaired Student’s t -tests were performed in each pulldown to determine statistical relevance. p vs. wild-type Arpp19 is shown; n = 3 biological independent samples for mutants D(78–95), D(96–111) and 2 A; n = 6 for the wild-type form and n = 4 for the 6A mutant. D Scheme depicting the two regions that have been deleted in the ‘inter-cassette’ motif. Table illustrating data on S67/S71 dephosphorylation timing as well as the capacity to bind B55 and to restore mitosis of the two mutants. Western blotting showing the amount of B55 present in the His-App19 pulldown assays of the indicated Arpp19 mutant forms. Graph bar representing the mean B55/Arpp19 ratio ± SD; n = 3 biological independent samples. E S67/S71 dephosphorylation assays of the indicated mutants of Arpp19 in kinase-inactivated extracts devoid or not of B55. Results were quantified and represented as the mean percentage of phosphorylation remaining ± SD; n = 3 biological independent samples. F A schematic of the DSG (D1) and the cassette (D2) regions of Arpp19 that have been exchanged in the D2-D1 mutant. A table with the dephosphorylation, binding and rescue results is also shown. The association of B55 to the D2-D1 mutant compared to the wild-type Arpp19 and the quantification of the mean B55/Arpp19 ± SD are shown. Two-tailed unpaired Student’s t -tests were performed in each pulldown to determine statistical relevance. p vs. wild-type Arpp19 is shown; n = 3 biological independent samples. G Dephosphorylation assay of S67/S71 of the wild type and the D2-D1 mutant in kinase-inactivated extracts that have been depleted or not of B55. Data from three experiments were represented as the mean percentage of phosphorylation remaining ± SD. n = 3 biological independent samples.

Techniques: Sequencing, De-Phosphorylation Assay, Western Blot, Phospho-proteomics, Two Tailed Test, Mutagenesis, Binding Assay

A Wild type and S109/S113D Arpp19 mutant were phosphorylated ‘in vitro’ with of [γ 33 P] ATP by GwlK72M on S67/S71 and supplemented to kinase-inactivated Xenopus egg extracts. The dephosphorylation of this residue was analysed at the indicated times by autoradiography ( P33 Arp) and the amount of Arpp19 in each sample measured by western blotting (Arp). B CytoStatic Factor (CSF) egg extracts were supplemented with a trace level of Arpp19-purified protein and activated to exit meiosis by the addition of active CamKII. The levels and dephosphorylation of the indicated proteins were analysed by western blotting, whereas Cyclin B/Cdk1 activity was measured by histone H1 phosphorylation (H1K). ‘Inter’ denotes interphase egg extracts. C As for C , except that S67/S71 Arpp19 dephosphorylation and PP2A-B55 reactivation upon meiosis exit in these extracts was blocked by the concomitant addition of GwlK72M-purified protein.

Journal: Nature Communications

Article Title: The study of the determinants controlling Arpp19 phosphatase-inhibitory activity reveals an Arpp19/PP2A-B55 feedback loop

doi: 10.1038/s41467-021-23657-0

Figure Lengend Snippet: A Wild type and S109/S113D Arpp19 mutant were phosphorylated ‘in vitro’ with of [γ 33 P] ATP by GwlK72M on S67/S71 and supplemented to kinase-inactivated Xenopus egg extracts. The dephosphorylation of this residue was analysed at the indicated times by autoradiography ( P33 Arp) and the amount of Arpp19 in each sample measured by western blotting (Arp). B CytoStatic Factor (CSF) egg extracts were supplemented with a trace level of Arpp19-purified protein and activated to exit meiosis by the addition of active CamKII. The levels and dephosphorylation of the indicated proteins were analysed by western blotting, whereas Cyclin B/Cdk1 activity was measured by histone H1 phosphorylation (H1K). ‘Inter’ denotes interphase egg extracts. C As for C , except that S67/S71 Arpp19 dephosphorylation and PP2A-B55 reactivation upon meiosis exit in these extracts was blocked by the concomitant addition of GwlK72M-purified protein.

Techniques: Mutagenesis, In Vitro, De-Phosphorylation Assay, Residue, Autoradiography, Western Blot, Purification, Activity Assay, Phospho-proteomics

A Arpp19 phosphorylated ‘in vitro’ in S67/S71 or S109/S113 residues by Gwl or PKA, respectively, were separately supplemented to kinase-inactivated Xenopus egg extracts and the dephosphorylation rate of each site, as well as the total amount of this protein were analysed by western blotting. B Arpp19 ‘in vitro’-phosphorylated by Gwl and Arpp19 ‘in vitro’-phosphorylated by PKA were mixed together into kinase-inactivated extracts and the dephosphorylation of each of these sites analysed at the indicated time points. C In a first run of dephosphorylation, a pulse of Arpp19 phosphorylated by PKA or by both PKA and Gwl was supplemented to kinase-inactivated extracts. Samples were then recovered at the indicated time points. After 12 min, a second round of dephosphorylation was performed in these extracts upon the re-addition of a new pulse of Arpp19 phosphorylated ‘in vitro’ by PKA on S109/S113. Phosphorylation of S67/S71 and S109/S113 in the samples were evaluated by western blotting with specific phospho-antibodies. D Prc1 was phosphorylated ‘in vitro’ by purified Cyclin A/Cdk and supplemented alone (left panels) or together with phospho-S67/S71 Arpp19 (right panels) to kinase-inactivated extracts and the phosphorylation of T481 of Prc1 and S67/S71 of Arpp19, as well as the amount of these two proteins were examined by western blotting. E Arpp19 phosphorylated ‘in vitro’ by PKA on S109/S113 and thio-phosphorylated on S67/S71 by GwlK72M were mixed into kinase-inactivated extracts. Dephosphorylation of these two residues were then measured at the indicated time points by western blotting. Data shown in the figure are representative of at least three different experiments.

Journal: Nature Communications

Article Title: The study of the determinants controlling Arpp19 phosphatase-inhibitory activity reveals an Arpp19/PP2A-B55 feedback loop

doi: 10.1038/s41467-021-23657-0

Figure Lengend Snippet: A Arpp19 phosphorylated ‘in vitro’ in S67/S71 or S109/S113 residues by Gwl or PKA, respectively, were separately supplemented to kinase-inactivated Xenopus egg extracts and the dephosphorylation rate of each site, as well as the total amount of this protein were analysed by western blotting. B Arpp19 ‘in vitro’-phosphorylated by Gwl and Arpp19 ‘in vitro’-phosphorylated by PKA were mixed together into kinase-inactivated extracts and the dephosphorylation of each of these sites analysed at the indicated time points. C In a first run of dephosphorylation, a pulse of Arpp19 phosphorylated by PKA or by both PKA and Gwl was supplemented to kinase-inactivated extracts. Samples were then recovered at the indicated time points. After 12 min, a second round of dephosphorylation was performed in these extracts upon the re-addition of a new pulse of Arpp19 phosphorylated ‘in vitro’ by PKA on S109/S113. Phosphorylation of S67/S71 and S109/S113 in the samples were evaluated by western blotting with specific phospho-antibodies. D Prc1 was phosphorylated ‘in vitro’ by purified Cyclin A/Cdk and supplemented alone (left panels) or together with phospho-S67/S71 Arpp19 (right panels) to kinase-inactivated extracts and the phosphorylation of T481 of Prc1 and S67/S71 of Arpp19, as well as the amount of these two proteins were examined by western blotting. E Arpp19 phosphorylated ‘in vitro’ by PKA on S109/S113 and thio-phosphorylated on S67/S71 by GwlK72M were mixed into kinase-inactivated extracts. Dephosphorylation of these two residues were then measured at the indicated time points by western blotting. Data shown in the figure are representative of at least three different experiments.

Techniques: In Vitro, De-Phosphorylation Assay, Western Blot, Phospho-proteomics, Purification

$A Kinase-inactivated Xenopus egg extracts were submitted to gel filtration chromatography and eluted fractions subsequently supplemented with Arpp19 phosphorylated ‘in vitro’ on either S109/S113 or S67/S71. The dephosphorylation of these residues and the amount of Arpp19 were then measured by western blotting (upper panels, dephosphorylation assay). ‘EX’ and 1 correspond to phosphorylation at time 0 and 10 minutes respectively, of the indicated residues upon directly mixing with a kinase-inactivated extract. The presence of the indicated proteins in elution fractions and in the kinase-inactivated extract sample were assessed by western blotting (lower panels, western blotting). Red lines highlight the fractions displaying S109/S113 and S67/S71 dephosphorylation activity. The name of the proteins whose level picked in these fractions are also depicted in red. B Prc1 ‘in vitro’ phosphorylated on T481 by purified Cyclin A/Cdk and Arpp19 phosphorylated on either S67/S71 or on S109/S113 were supplemented together or separately to kinase-inactivated extracts depleted of B55 and the dephosphorylation of the corresponding residues analysed over the time by western blotting. Data of the figure are confirmed in three different experiments.$

Journal: Nature Communications

Article Title: The study of the determinants controlling Arpp19 phosphatase-inhibitory activity reveals an Arpp19/PP2A-B55 feedback loop

doi: 10.1038/s41467-021-23657-0

Figure Lengend Snippet: A Kinase-inactivated Xenopus egg extracts were submitted to gel filtration chromatography and eluted fractions subsequently supplemented with Arpp19 phosphorylated ‘in vitro’ on either S109/S113 or S67/S71. The dephosphorylation of these residues and the amount of Arpp19 were then measured by western blotting (upper panels, dephosphorylation assay). ‘EX’ and 1 correspond to phosphorylation at time 0 and 10 minutes respectively, of the indicated residues upon directly mixing with a kinase-inactivated extract. The presence of the indicated proteins in elution fractions and in the kinase-inactivated extract sample were assessed by western blotting (lower panels, western blotting). Red lines highlight the fractions displaying S109/S113 and S67/S71 dephosphorylation activity. The name of the proteins whose level picked in these fractions are also depicted in red. B Prc1 ‘in vitro’ phosphorylated on T481 by purified Cyclin A/Cdk and Arpp19 phosphorylated on either S67/S71 or on S109/S113 were supplemented together or separately to kinase-inactivated extracts depleted of B55 and the dephosphorylation of the corresponding residues analysed over the time by western blotting. Data of the figure are confirmed in three different experiments.

Techniques: Filtration, Chromatography, In Vitro, De-Phosphorylation Assay, Western Blot, Phospho-proteomics, Activity Assay, Purification

A Arpp19 was phosphorylated ‘in vitro’ on S109/S113, S67/S71 or thio-phosphorylated on S67/S71, supplemented alone or with the indicated combinations to kinase-inactivated extracts and the temporal pattern of dephosphorylation analysed. B ENSA protein was phosphorylated ‘in vitro’ on S67 by GwlK72M or S109 by PKA and supplemented alone or combined as indicated to kinase-inactivated extracts and the dephosphorylation time of these residues, as well as the amount of ENSA protein examined. C Phospho-S109 ENSA and phospho-S67/S71 Arpp19 or phospho-S109/S113 Arpp19 and phospho-S67 ENSA were simultaneously added into kinase-inactivated extracts and the timing of dephosphorylation of the different phospho-sites analysed. Experiments supporting the data of this figure were performed at least three times.

Journal: Nature Communications

Article Title: The study of the determinants controlling Arpp19 phosphatase-inhibitory activity reveals an Arpp19/PP2A-B55 feedback loop

doi: 10.1038/s41467-021-23657-0

Figure Lengend Snippet: A Arpp19 was phosphorylated ‘in vitro’ on S109/S113, S67/S71 or thio-phosphorylated on S67/S71, supplemented alone or with the indicated combinations to kinase-inactivated extracts and the temporal pattern of dephosphorylation analysed. B ENSA protein was phosphorylated ‘in vitro’ on S67 by GwlK72M or S109 by PKA and supplemented alone or combined as indicated to kinase-inactivated extracts and the dephosphorylation time of these residues, as well as the amount of ENSA protein examined. C Phospho-S109 ENSA and phospho-S67/S71 Arpp19 or phospho-S109/S113 Arpp19 and phospho-S67 ENSA were simultaneously added into kinase-inactivated extracts and the timing of dephosphorylation of the different phospho-sites analysed. Experiments supporting the data of this figure were performed at least three times.

Techniques: In Vitro, De-Phosphorylation Assay

A The docking of phospho-S67/S71 of Arpp19 into PP2A catalytic subunit (C) as an inhibitor conformation, its rearrangement towards a substrate conformation and its final dissociation upon dephosphorylation. Phosphorylation of S67/S71 is represented (yellow circle). The two aspartic residues D70 and D73 (pink circles) that could interact with the metallic centre (red circles) and with the two arginine residues (R) of the PP2A C subunit are shown. The putative effects of KKR/AAA, G72A, and D70A/D73A mutants of Arpp19 on the docking conformation of phospho-S67/S71 are indicated. B Putative docking of the DSG motif of Arpp19 into the active site of PP2A-B55 (PDB2IE4) . Left: conformation of the peptide orientating the phosphorylated serine (yellow circle) toward the catalytic centre with the two aspartates (red circles) pointing toward nearby arginines (R89 and R214). Right: inhibited form in which the phosphorylated serine points outside (toward R268), while the dications (violet spheres) from the catalytic centre are chelated by one or two aspartates from the DSG motif.

Journal: Nature Communications

Article Title: The study of the determinants controlling Arpp19 phosphatase-inhibitory activity reveals an Arpp19/PP2A-B55 feedback loop

doi: 10.1038/s41467-021-23657-0

Figure Lengend Snippet: A The docking of phospho-S67/S71 of Arpp19 into PP2A catalytic subunit (C) as an inhibitor conformation, its rearrangement towards a substrate conformation and its final dissociation upon dephosphorylation. Phosphorylation of S67/S71 is represented (yellow circle). The two aspartic residues D70 and D73 (pink circles) that could interact with the metallic centre (red circles) and with the two arginine residues (R) of the PP2A C subunit are shown. The putative effects of KKR/AAA, G72A, and D70A/D73A mutants of Arpp19 on the docking conformation of phospho-S67/S71 are indicated. B Putative docking of the DSG motif of Arpp19 into the active site of PP2A-B55 (PDB2IE4) . Left: conformation of the peptide orientating the phosphorylated serine (yellow circle) toward the catalytic centre with the two aspartates (red circles) pointing toward nearby arginines (R89 and R214). Right: inhibited form in which the phosphorylated serine points outside (toward R268), while the dications (violet spheres) from the catalytic centre are chelated by one or two aspartates from the DSG motif.

Techniques: De-Phosphorylation Assay, Phospho-proteomics

Journal: Current biology : CB

Article Title: Bistable, Biphasic Regulation of PP2A-B55 Accounts for the Dynamics of Mitotic Substrate Phosphorylation

doi: 10.1016/j.cub.2020.11.058

Figure Lengend Snippet:

Article Snippet: The following antibodies were used for detection of the respective proteins: mouse α-Cdc27 (BD Biosciences, #610455), mouse α-cyclin B2 (Santa Cruz Biotechnology, #sc-53239), rabbit α-Nup53 serum, rabbit α-PPP1A pT320 (Abcam, #ab62334), rabbit α-Cdc25C, rabbit α-Wee1 pT150, rabbit α-Greatwall serum, rabbit α-ENSA serum, rabbit α-Arpp19, rabbit α-ENSA pS67/Arpp19 pS62 (Cell Signaling Technology, #5240) and rabbit α-Cdk1 pY15 (Cell Signaling Technology, #9111L).

Techniques: Recombinant, Protease Inhibitor, Expressing, Software

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rabbit anti phospho ensa (Cell Signaling Technology Inc)

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